【摘要】目的 綜述國(guó)內(nèi)外有關(guān)針刺戒毒的臨床療效和機(jī)理 的進(jìn)展。方法 總結(jié)了耳針、體針、耳體結(jié)合舉治療方法在臨床運(yùn)用中的療效，并簡(jiǎn)要闡述了針刺戒毒的可能機(jī)制。結(jié)果 為針刺戒毒的臨床運(yùn)用提供了可借鑒的資料。結(jié)論 針刺戒毒具有特效、安全、價(jià)廉、無(wú)明顯副作用的特點(diǎn)。
[Abstract] Objective The author reviewed the advances of the clinical effect of acupuncture and its mechanism in stopping drug abuse in recent years. Method The author summarized the curative effect of clinical application of various needling methods， such as auricular acupuncture, body acupuncture and the combination of auricular and body acupuncture. The possibility of stopping drug abuse by acupuncture was expounded. Result To provide necessary data for the clinical application of acupuncture to stop drug abuse. Conclusion In stopping drug abuse, acupuncture therapy is highly effective，safe，cheap and free of side effect.
摘要 目的：從大鼠脾臟克隆信號(hào)分子guanine-nueleotide-releastng factor (GRF) pleckstrin homofogy (PH)結(jié)構(gòu)域基因并迸行谷胱肽轉(zhuǎn)移酶融合表達(dá)，為進(jìn)一步尋找其新配基、研究其功 能打下基礎(chǔ)。方法：采用一步法從大鼠新鮮脾組織中提取反轉(zhuǎn)錄合成及方法擴(kuò)大增目的基因片段，并克隆到pUC19中。引物末端標(biāo)記后，以雙氧終止法測(cè)序。然后再克隆到表達(dá)載體PGEX-4T-1中作GST融合表達(dá)。結(jié)果:信號(hào)GRF的PH結(jié)構(gòu)域基因完全正確，內(nèi)部不含終止碼。并在表達(dá)載體pGEX-4T-1中獲得融合表達(dá)。結(jié)論：從組織細(xì)胞中克隆Ph結(jié)構(gòu)域基因是可 行的方法，可在大腸桿菌中以GST融合蛋白的形式表達(dá)。
Abstract AIM: To screen for novel ligands of the PH domains and to investigate the functions of the PH domains in signal transduction. METHODS: Rat spleens were employed to clone the gene encoding PH domain of GRF. Total RNA molecules were isolated from fresh sheens and mRNAs were reversely transcriped into cDNAs. After amplication by PCR, the fragments of DNA were cloned into vector pUC19. The method of dideoxy-termination was employed to sequence with labelled primmers, and then the gene was cloned into vector pGEX-4T-1 ?o express in fusion with GST. RESULTS：The sequence of gene was correct and the gene was successfully expressed in pGEX-4T-1. CONCLUSION: The methods of cloning PH domains of signaling molecules are feasible and useful in study of signal trans-duction. The fragments of the gene can correctly be expressed in E, Coli as fusion with GST.
